Lipase PL from Alcaligenes sp. Triacylglycerol lipase EC 3.1.1.3

Enzymatic features

Lipase PL is active and stable in various organic solvents and suitable for synthesis of esters and transesterification. The activity of Lipase PL is remarkably stimulated in the presence of bile salts like pancreatic lipases.

Specifications & Characteristics

Appearance		Beige powder
Activity		ca. 100,000 U/g powder
Molecular weight		30,000 (SDS-PAGE)
Isoelectric point		4.5
Optimum pH		8 - 9.5
pH stability		7 - 10
Optimum temperature		50 ºC
Thermal stability		Below 40 ºC
Inhibitors		Cationic detergents
Specificities	Positional specificity	preferentially hydrolyzing the sn-1 and sn-3 positions of triacylglycerols.
	Activity on esters	ca. 75 U/g (p-Nitrophenyl acetate as a substrate)
Storage		Desiccate and store below 25 ºC