Lipase SL from Pseudomonas (Burkholderia) cepacia Triacylglycerol lipase EC 3.1.1.3

Enzymatic features

Lipase SL has high activities over a wide range of pH values (pH 3-10) and temperatures (below 80 ºC;), and shows a wide specificity towards a variety of esters such as p-nitrophenyl esters as well as triacylglycerols.

Specifications & Characteristics

Appearance		Beige powder
Activity		ca. 45,000 U/g powder
Molecular weight		31,000 (SDS-PAGE)
Isoelectric point		5.2
Optimum pH		7 - 9
pH stability		6 - 10
Optimum temperature		65 - 70 ºC
Thermal stability		Below 60 ºC
Inhibitors		Cationic detergents
Activators		Nonionic detergents
Specificities	Positional specificity	preferentially hydrolyzing the sn-1 and sn-3 positions of triacylglycerols.
	Activity on esters	ca. 700 U/g (p-Nitrophenyl acetate as a substrate)
Storage		Desiccate and store below 25 ºC