Lipase TL from Pseudomonas stutzeri Triacylglycerol lipase EC 3.1.1.3

Enzymatic features

Lipase TL has high activities on cholesterol esters and can be used to determine concentration of cholesterol esters. Lipase TL hardly hydrolyzes esters of polyunsaturated fatty acids and enables the enrichment of acylglycerols containing polyunsaturated fatty acids.

Specifications & Characteristics

Appearance		Beige powder
Activity		ca. 50,000 U/g powder
Molecular weight		27,000 (SDS-PAGE)
Isoelectric point		6.6
Optimum pH		7 - 8
pH stability		6 - 9
Optimum temperature		50 ºC
Thermal stability		Below 40 ºC
Inhibitors		Cationic detergents, EDTA
Specificities	Positional specificity	preferentially hydrolyzing the sn-1 and sn-3 positions of triacylglycerols.
	Activity on esters	ca. 650 U/g (p-Nitrophenyl acetate as a substrate)
Storage		Desiccate and store below 25 ºC